Composite

Part:BBa_K676777

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)


GyrA cassette

This expression cassette is regulated by an IPTG inducible promoter and will be expressing Gyr A subunits . Gyr A subunit will bind to the DNA ( gyrase binding site) and is responsible for catalysing DNA breakage and resealing in order to change the level of supercoiling in DNA. However in order to introduce negative supercoiling into the DNA , a functional tetrameric gyrase structure with two GyrA and two GyrB should form with the presence of divalent magnesium ions and ATP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None